Saturday, February 18, 2012

OBJ 5.13a Recombinant DNA5.13b Hosting recombinant DNA

Describe how plasmids and viruses can act as vectors, which take up pieces of DNA, then insert this recombinant DNA into other cells.
Recombinant DNA = a section of DNA is snipped out of the DNA of one species and inserted into the DNA of another the new DNA is called the "Recombinant DNA." 

1. Plasmid
  • found in bacterial cells 
  • small circular ring of DNA 
  • don't carry very many genes 
2. Virus = 
  • has a protein shell called a Caspid
  • contains a nucleic acid (DNA/RNA) 
  • (no cytoplasm, no nucleus etc)

    3.  Human Chromosome 
  • (length of DNA) 
  • identify a gene --> this gene codes for the protein Insulin (hormone controlling blood sugar levels) 
  • the restriction enzyme is selected to cut the DNA (to cut the gene for insulin) 
  • the gene is cut --> now isolate the plasmids and cut it with the same srestriction enzyme
  • the plasmids are now open in a structure much like a "C"
  • the opened up plasmids and the human gene insulin are mixed with a DNA ligase enzyme to create recombinant plasmids (combination of human gene + plasmid)

1 comment:

  1. These lessons are very helpful.
    What course it it from? Is is the IGCSE course?