Understand that large amounts of human insulin can be manufactured from genetically modified bacteria that are grown in a fermenter
1. Bacterial cell transformed by recombinant DNA (with the plasmid DNA combined with the human DNA of the insulin gene.)
2. A culture of this bacteria (large population) is injected into a fermenter
Necessities :
- provide nutrients
- control the temperature + pH
- control the gases in the fermenting chamber
Creating the optimum temperature for bacterial growth ---> pop will increase + bacteria will manufacture the protein Insulin.
3. The bacteria will manufacture the insulin protein from the nutrient protein (amino acid)
4. Then it will be necessary to remove the product --> carry out purification by downstream processing.
5. The genetically engineered human insulin is called 'Humulin.'
1. Bacterial cell transformed by recombinant DNA (with the plasmid DNA combined with the human DNA of the insulin gene.)
2. A culture of this bacteria (large population) is injected into a fermenter
Necessities :
- provide nutrients
- control the temperature + pH
- control the gases in the fermenting chamber
Creating the optimum temperature for bacterial growth ---> pop will increase + bacteria will manufacture the protein Insulin.
3. The bacteria will manufacture the insulin protein from the nutrient protein (amino acid)
4. Then it will be necessary to remove the product --> carry out purification by downstream processing.
5. The genetically engineered human insulin is called 'Humulin.'
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