Describe the stages in the production of cloned mammals involving the introduction of a
diploid nucleus from a mature cell into an enucleated egg cell, illustrated by Dolly the
sheep.
Dolly the sheep:
1. An egg cell was removed from the ovary of an adult female sheep and its nucleus removed. (enucleated)
2. The nucleus from an udder cell of a donor sheep was inserted into the empty egg cell.
3. The fused cell then began to develop normally, using genetic information from the donated DNA
4. The cell develops into an embryo by mitosis, and is implanted into the uterus of a foster mother sheep.
5. Result = lamb born (Dolly) genetically identical to 'parent' sheep.
Dolly the sheep:
1. An egg cell was removed from the ovary of an adult female sheep and its nucleus removed. (enucleated)
2. The nucleus from an udder cell of a donor sheep was inserted into the empty egg cell.
3. The fused cell then began to develop normally, using genetic information from the donated DNA
4. The cell develops into an embryo by mitosis, and is implanted into the uterus of a foster mother sheep.
5. Result = lamb born (Dolly) genetically identical to 'parent' sheep.
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